Publications

  1. Neidleman J., Luo X., McGregor M., Xie G., Murray V., Greene W.C., Lee S.A., Roan N.R. mRNA vaccine-induced SARS-CoV-2-specific T cells recognize B.1.1.7 and B.1.351 variants but differ in longevity and homing properties depending on prior infection status. 2021. bioRxiv preprint.
  2. Peters B.A., Moon J., Hanna D.B., Kutsch O., Fischl M., Moran C.A., Adimora A.A., Gange S., Roan N.R., Michel K.G., Augenbraun M., Sharma A., Landay A., Desai S., Kaplan R.C. T-cell immune dysregulation and mortality in women with HIV. 2021. Journal of Infectious Diseases
  3. Ma T. Ryu H., McGregor M., Babcock B., Neidleman J., Xie G., George A.F., Frouard J., Murray V., Gill G., Ghosn E., Newell E., Lee S., Roan N.R. Protracted yet coordinated differentiation of long-lived SARAS-CoV-2-specific CD8+ T cells during COVID-19 convalescence. 2021. Journal of Immunology.

  4. Moron-Lopez S., Xie G., Kim P., Siegel D.A., Lee S., Wong J.K., Price J.C., Elnachef N., Greenblatt R.M., Tien P.T., Roan N.R., Yukl S.A. Tissue-specific differences in HIV DNA levels and mechanisms that govern HIV transcription in blood, gut, genital tract, and liver in ART-treated women. 2021. Journal of the International AIDS Society.

  5. Neidleman J., Luo X., George A.F., McGregor M., Yang J., Yun C., Murray V., Gill G., Greene W.C., Vasquez J., Lee S., Ghosn E., Lyncch K., Roan N.R. Distinctive features of SARS-CoV-2-specific T cells predict recovery from severe COVID-19. 2021. Cell Reports

  6. Egedal J.H., Xie G., Packard T.A., Laustern A., Neidleman J. Georgiou K., Pillai S., Greene W.C., Jakobsen M.R., Roan N.R. Hyaluronic acid is a negative regulator of mucosal fibroblast-mediated enhancement of HIV infection. 2021. Mucosal Immunology.

  7. Xie G., Luo X., Ma T., Frouard J., Neidleman J., Hoh R., Deeks S.G., Greene, W.C. Roan N.R. Characterization of HIV-induced remodeling reveals differences in infection susceptibility of memory CD4+ T cell subsets in vivo. 2021. Cell Reports, 35(4):109038.

  8. Gramatica A., Schwarzer R., Brantley W., Varco-Merth B., Sperber H., Hull P., Montano M., Migueles S., Rosenthal D., Hogan L., Johnson J., Packard T., Grimmett Z., Herzig E., Besnard E., Nekorchuk M., Hsiao F., Deeks S., Snape M., Kiernan B., Roan N.R., Lifson J., Estes J., Picker L., Verdin E., Krogan N., Henrich T., Connors M., Ott M., Pillai S., Okoye A., Greene W.C. Evaluating a new class of AKT/mTOR activators for HIV latency reversing activity ex vivo and in vivo. 2021. Journal of Virology, JVI.0293-20.

  9. McGinnis C.S., Siegel D.A., Xie G., Hartoularos G. Stone M., Ye C.J., Gartnenr Z.J., Roan N.R.*, Lee, S.A.* No detectable alloreactive transcriptional responses under standard sample preparation conditions during donor-multiplexed single-cell RNA sequencing of peripheral blood mononuclear cells. *Co-corresponding authors. 2021. BMC Biology, 19(1):10.

  10. Yukl S.A., Khan S., Chen T.H., Trapecar M., Wu F., Xie G., Telwatte S., Fulop D., Pico A.R., Laird G.M., Ritter K.D., Jones N.G., Lu C.M., Siliciano R.F., Roan N.R., Milush J.M., Somsouk M., Deeks S.G., Hunt P.W., Sanjabi S. Shared mechanisms govern HIV transcriptional suppression in circulating CD103+ and gut CD4+ T cells. 2020. Journal of Virology, 95(2):e01331-20.

  11. Warren J.A., Zhou S., Xu Y., Moeser M.J., MacMilan D.R., Council O., Kirchherr J., Sung J.M., Roan N.R., Adimora A.A., Joseph S., Kuruk j.D., Gay C.L., Margolis D.M., Archin N., Brumme Z.L., Swanstrom R., Goonetilleke N. The HIV-1 latent reservoir is largely sensitive to circulating T cells. 2020. eLife, doi:10.7554/eLife.57246.

  12. Seidman D., Cavrois M., Hilton J.F., Roan N.R., Averbach S., Takeda M., Chang E., Raman N., Greenblatt R., Shacklett B.L., Smith-McCune K. Reproductive tract immune cells from pregnant women or those u sing depot dedroxyprogesterone acetate show no excess susceptibility to HIV-1: results of an ex vivo fusion assay. 2020. Contraception, S0010-7824(20)30343-7.

  13. Neidleman J., ., Luo X., Frouard J., Xie G., Hsiao F., Ma T., Morcilla V., Lee A., Telwatte S., Thomas R., Tamaki W., Wheeler B., Hoh R., Somsouk M., Vohra P., Milush J., James K., Archin N.M., Hunt P.W., Deeks S.G., Yukl S.A., Palmer S., Greene W.C., Roan N.R. Phenotypic analysis of the unstimulated in vivo HIV CD4 T cell reservoir. 2020. Elife, 9:e60933.

  14. Neidleman J., Luo X., Frouard J., Xie G., Gill G., Stein E.S., McGregor M., Ma T., George A.F., Kosters A., Greene W.C., Vasquez J., Ghosn E., Lee S., Roan N.R. SARS-CoV-2-specific T cells exhibit phenotypic features reflecting robust helper function, lack of terminal differentiation, and high proliferative potential. 2020. Cell Reports Medicine, Aug 19:100081.

  15. Xu-Fuery S.C., LoRicco J.G., Bishop, A.C., James N.A., Huynh W.H., MaCallum S.A., Roan N.R., Makhatadze G.I. Sequence-independent recognition of the amyloid structural motif by GFP protein family. 2020. Proceedings of the National Academy of Sciences U S A, Aug24:202001457.

  16. Colomb F., Giron L.B., Kuri-Cervantes L., Adeniji O.S., Ma T., Dweep H., Battivelli E., Verdin E., Palmer C.S., Tateno H., Kossenkov A.V., Roan N.R., Betts M.R., Abdel-Mohsen M. Sialyl-LewisX Glycoantigen Is Enriched on Cells with Persistent HIV Transcription during Therapy. 2020. Cell Reports, 32(5):107991.

  17. Falcinelli S.D., Shook-Sa B.E., Dewey M.G., Sridhar S., Read J., Kirchher J., James K.S., Allard B., Ghofrani S., Stuelke E., Baker C., Roan N.R., Eron J.J., Kuruc J.D., Ramirez C., Gay C., Mollan K.R., Margolis D.M., Adimora A.A., Archin N.M. Impact of biological sex on immune activation and frequency of latent HIV reservoir during suppressive antiretroviral therapy. 2020. Journal of Infectious Diseases, jiaa298.

  18. Ma T., Luo X., George A., Mukherjee G., Sen N., Sptizer T., Giudice L.C., Greene W.C., Roan N.R. HIV efficiently infects T cells from the endometrium and remodels them to promote systemic viral spread. 2020. ELife, 9:e55487.

  19. Marques de Menezes E.G., Jang K., George A.F., Nyegaard M., Neidleman J., Inglis H.C., Danesh A., Deng X., Afshari A., Kim Y.H., Billaud J., Marson K., Pilcher C.D., Pillai S.K., Norris P.J., Roan N.R. Seminal plasma-derived extracellular vesicle fractions from HIV-infected men exhibit unique miRNA signatures and induce a pro-inflammatory response in cells isolated from the female reproductive tract. 2020. Journal of Virology, 94(16):e00525.

  20. Hsiao F., Frouard J., Gramatica A., Guorui X., Telwatte S., Lee G.Q., Roychoudhury P., Schwarzer R., Luo X., Yukl S.A., Lee S., Hoh R., Deeks S.G., Jones R.B., Cavrois M., Greene W.C., Roan N.R. Tissue memory CD4+ T cells expressing the IL-7 receptor-alpha (CD127) preferentially support latent HIV-1 infection. 2020. PLoS Pathogens, 16(4):e1008450.

  21. George A.F., Jang K.S., Nyegaard M., Neidleman J., Spitzer T.L., Xie G., Chen J.C., Herzig E., Laustsen A., Marques de Menezes E.G., Houshdaran S., Pilcher C.D., Norris P.J., Jakobsen M.R., Greene W.C., Giudice L.C., Roan N.R. Seminal plasma promotes decidualization of endometrial stromal fibroblasts in vitro from women with and without inflammatory disorders in a manner dependent on interleukin-11 signaling. 2020. Hum Reprod, 10.1093/humrep/deaa015.

  22. Thomas A., Hammarlund E., Gao L., Holman S., Michel K.G., Glesby M., Villacres M.C., Golub E.T., Roan N.R., French A.L., Augenbraun M.H., Slifka M.K. Loss of pre-existing immunological memory among HIV patients despite immune reconstitution with antiretroviral therapy. 2019. Journal of Infectious Diseases, 222(2):243.

  23. Sholtis K., T. Sholtis K., Trumble I., Clohosey M., Moeser M., Roan N.R., Adimora A.A., Joseph S.B., Archin N.M., Hudgens M., Soriano-Sarabia N. Measuring the contribution of gd T cells to the persistent HIV reservoir. 2019. AIDS, 34(3):363.

  24. Cavrois M., Hilton J.F., Roan N.R., Takeda M., Seidman D., Averbach S., Chang E., Raman N., Greenblatt R., Shacklett B.L. Smith-McCune K. Effects of the levonorgestrel-containing device, copper intrauterine device, and levonogestrel-containing oral contraceptive on susceptibility of immune cells from cervix, endometrium and blood to HIV-1 fusion measured ex vivo. 2019. PLoS One, 14(8):e0221181.
  25. Skinner W.M., Mannowetz N., Lishko P.V., Roan N.R. Single-cell motility analysis of tethered human spermatozoa. 2019. Bio-protocol, 9(5): e3182.
  26. Lausten A., Bak R.O., Krapp, C., Kjaer L., Egedahl J.H., Petersen C.C., Pillai S., Tang H.Q., Uldbjerg N., Porteus M., Roan N.R., Nyegaard M., Denton P.W., Jakobsen M.R. Interferon priming is essential for human CD34+ cell-derived plasmacytoid dendritic cell maturation and function. 2018. Nature Communications, 30;9(1):3525. 
  27. Müller J.A., Harms M., Krüger F., Groß R., Joas S., Hayn M., Dietz A.N., Lippold S., von Einem J., Schubert A., Michel M., Mayer B., Cortese M., Jang K.S., Sandi-Monroy N., Deniz M., Ebner F., Vapalahti O., Otto M., Bartenschlager R., Herbeuval JP, Schmidt-Chanasit J., Roan N.R., Münch J. Semen inhibits Zika virus infection of cells and tissues from the anogenital region. 2018. Nature Communications, 9(1):2007.
  28. Röcker A., Roan N.R., Yadav J.K., Fändrich M., Münch J. 2018. Structure, function and antagonism of semen amyloids. Chemical Communications, 54(55):7557-7569.
  29. Roan N.R. Resident T cells stand up to HIV. 2018. Science Immunology Focus article. 2018. 3(24) pii: eaat6121.
  30. Martin J.W., Chen J.C., Neidleman J., Tatsumi K., Hu J., Giudice L.C., Greene W.C., Roan N.R. Potent and rapid activation of tropomyosin-receptor kinase A in endometrial stromal fibroblasts by seminal plasma. 2018. Biology of Reproduction, 99(2):336-348.
  31. Trepecar M., Khan S., Roan N.R., Chen T.H., Telwatte S., Deswal M., Pao M., Somsouk M., Deeks S.G., Hunt P.W., Yukl S., Sanjabi S. 2017. An optimized and validated method for isolation and characterization of lymphocytes from HIV+ human gut biopsies. AIDS and Human Retroviruses 33(S1):S31-S39.
  32. Cavrois M., Banerjee T., Mukherjee G., Raman N., Hussien R., Rodriguez B.A., Vasquez J., Spitzer M.H., Lazarus N.H., Jones J.J., Ochsenbauer C., McCune J.M., Butcher E.C., Arvin A.M., Sen N., Greene W.C., Roan N.R. Mass cytometric analysis of HIV entry, replication, and remodeling in tissue CD4+ T cells. 2017. Cell Reports 20(4):984-998.
  33. Roan N.R., Sandi-Monroy N., Kohgadai N., Usmani S.M., Hamil K.G., Neidleman J., Montano M., Standker L., Rocker A., Cavrois M., Rosen J., Marson K., Smith J.F., Pilcher C.D., Gagsteiger F., Sakk O., O’Rand M., Lishko P.V., Kirchhoff F., Münch J., Greene W.C. Semen amyloids participate in human spermatozoa selection and clearance. 2017. Elife 6:e24888.
  34. Neidleman J.A., Chen J.C., Kohgadai N., Müller J.A., Laustsen A., Thavachelvam K., Jang K.S., Stürzel C.M., Jones J.J., Ochsenbauer C., Chitre A., Somsouk M., Garcia M.M., Smith J.F., Greenblatt R.M., Münch J., Jakobsen M.R., Giudice L.C., Greene W.C., Roan N.R. Mucosal stromal fibroblasts markedly enhance HIV infection of CD4+ T cells. 2017. PLoS Pathogens 13(2):e1006163.
  35. LoRicco J.G., Xu C.S., Neidleman J., Bergkvist M., Greene W.C., Roan N.R.*, Makhatadze G.I.* Gallic acid is an antagonist of semen amyloid fibrils that enhance HIV-1 infection. *Co-corresponding authors. 2016. J Biol Chem, 291(27):14045.
  36. Bergman P., Roan N.R., Römling U., Bevins C.L., Münch J. Amyloid formation: functional friend or fearful foe? 2016. J Intern Med, 280(2):139.
  37. Müller J.A.*, Zirafi O.*, Roan N.R., Lee S.J., Münch J. Evaluation of EPI-X4 as a urinary peptide biomarker for diagnosis and prognosis of late acute GVHD. 2016. *Authors contributed equally to work. 2016. Bone Marrow Transplantation, 51(8):1137.
  38. Roan N.R. and Jakobsen M.R. Friend or foe: Innate sensing of HIV in the female reproductive tract. 2016. Curr HIV/AIDS Rep, 13(1):53.
  39. Camus C., Matusali G., Bourry O., Mahe D., Aubry F., Bujan L., Pasquier C., Massip P.,Ravel C., Zirafi O., Münch J, Roan N.R., Pineau C., Dejucq-Rainsford N. Comparison of the effect of semen from HIV-infected and uninfected men on CD4+ T cell infection. 2016. AIDS, 30(8):1197.
  40. Roan N.R. and Münch J. Improving preclinical models of HIV microbicide efficacy. 2015. Trends in Microbiology, 23(8):445.
  41. Chen J.C., Roan N.R. Isolation and culture of human endometrial epithelial cells and stromal fibroblasts. 2015. Bio Protocols, 5(20). Pii: 31623.
  42. Zirafi O.*, Kim K.A.*, Roan N.R.*, Kluge S.F., Müller J.A., Jiang S., Mayer B., Greene W.C., Kirchhoff F., Münch J. 2014. *Authors contributed equally to work. Semen enhances HIV infectivity and impairs the antiviral efficacy of microbicides. Science Translational Medicine, 6(262):262ra157.
  43. French K.C., Roan N.R., Makhatadze G.I. 2014. Structural characterization of semen coagulum-derived SEM1(86–107) amyloid fibrils that enhance HIV-1 infection. Biochemistry, 53(20):3267-77.
  44. Roan N.R., Liu H., Usmani S.M., Neidleman J., Müller A.J., Avila-Herrera A., Gawanbacht A., Zirafi O., Chu S., Dong M., Kumar S.T., Smith J.F., Pollard K.S., Fändrich M., Kirchhoff F., Münch J., Witkowska H.E., Greene W.C. 2014. Liquefaction of semen generates and later degrades a conserved semenogelin peptide that enhances HIV infection. Journal of Virology, 88(13):7221-7234.
  45. Roan N.R., Chu S., Liu H., Neidleman, J., Witkowska, H.E., Greene, W.C. 2014. The interaction of fibronectin with semen amyloids synergistically enhances HIV infection. Journal of Infectious Diseases, 210(7):1062-6.
  46. Chen J.C., Johnson B.A., Erikson D.W., Piltonen T.T., Barragan F., Chu S., Kohgadai N., Irwin J.C., Greene W.C., Giudice L.C., Roan N.R. 2014. Seminal plasma induced global transcriptomic changes associated with cell migration, proliferation, and viability in endometrial epithelial cells and stromal fibroblasts. Human Reproduction, 29(6):1255-70.
  47. Usmani S.M., Zirafi O.Z., Müller A.J., Sandi-Monroy N., Yadav J.K., Meier C., Weil T., Roan N.R., Greene W.C., Walther P., Nilsson K.P.R., Hammarström P., Wetzel R., Pilcher C.D., Gagsteiger F., Fändrich M., Kirchhoff F., Münch J. 2014. Direct Visualization of HIV-enhancing endogenous amyloid fibrils in human semen. Nature Communications, 5:3508.
  48. Tang Q., Roan N.R., Yamamura Y. 2013. Seminal plasma and semen amyloids enhance cytomegalovirus infection in cell culture. Journal of Virology 87(23):12583-91.
  49. Yolamanova M., Meier C., Shaytan A.K., Vas V., Bertoncini C.W., Arnold F., Zirafi O., Usmani S.M., Müller J.A., Sauter D., Goffinet C., Palesch D., Walther P., Roan N.R., Geiger H., Lunov O., Simmet T., Bohne J., Schrezenmeier H., Schwarz K., Ständker L., Forssmann W.G., Salvatella X., Khalatur P.G., Khokhlov A.R., Knowles T.P., Weil T., Kirchhoff F., Münch J. 2013. Peptide nanofibrils boost retroviral gene transfer and provide a rapid means for concentrating viruses. Nature Nanotechnology 8(2):130-6.
  50. Roan N.R., Cavrois M., Greene W.C. 2012. [Role of semen-derived amyloid fibrils as facilitators of HIV infection]. Med Sci (Paris), 28(4): 358-60.
  51. Arnold F., Schnell J., Zirafi O., Sturzel C., Meier C., Weil T., Standker L., Forssmann W.G., Roan N.R., Greene W.C., Kirchhoff F., Munch J. 2012. Naturally occurring fragments from two distinct regions of the prostatic acid phosphatase form amyloidogenic enhancers of HIV infection. Journal of Virology, 86(2)1244-9.
  52. Roan N.R., Müller A.J., Liu H., Chu S., Arnold F., Stürzel C., Walther P., Dong M., Witkowski, E., Kirchhoff, F., Münch J., Greene, W.C. 2011. Peptides released by physiological cleavage of semen coagulum proteins form amyloids that enhance HIV infection. Cell Host and Microbe 10: 541-550.
  53. Kim K.A., Yolamanova M., Zirafi O., Roan N.R., Standker L., Forssmann W.G., Burgener A., Dejucq-Rainsford N., Hahn B.H., Shaw G.M., Greene W.C., Kirchhoff F., Münch J. 2010. Semen-mediated enhancement of HIV infection is donor-dependent and correlates with the levels of SEVI. Retrovirology 7:55.
  54. Roan N.R., Sowinski, S., Münch J., Kirchhoff F., Greene, W.C. 2010. Aminoquinoline surfen inhibits the action of SEVI (semen-derived enhancer of viral infection). Journal of Biological Chemistry 285: 1861-9.
  55. Gondek D.C., Roan N.R., Starnbach M.N. 2009. T cell responses in the absence of IFN-g exacerbate uterine infection with Chlamydia trachomatis. Journal of Immunology 183: 1313-9.
  56. Roan N.R., Münch J., Arhel N., Mothes W., Neidleman J., Kobayashi A., Smith-McCune K., Kirchhoff F., Greene W.C. 2009. The cationic properties of SEVI underlie its ability to enhance HIV infection. Journal of Virology 83: 73-80 cover article.
  57. Starnbach M.N., Roan N.R. 2008. Conquering sexually transmitted diseases. Nature Reviews Immunology 8: 313-7.
  58. Grotenbreg G.M.*, Roan N.R.*, Guillen E., Meijers R., Wang, J., Bell, G.W., Starnbach, M.N., Ploegh, H.L. 2008. Discovery of CD8+ T cell epitopes in Chlamydia trachomatis infection through use of caged class I MHC tetramers. *Authors contributed equally to work. Proceedings of the National Academy of Sciences U S A. 105:3831-6.
  59. Roan N.R., Starnbach, M.N. 2008. Immune-mediated control of Chlamydia infection. Cellular Microbiology 10: 9-19.
  60. Roan N.R., Greene, W.C. 2007. A seminal finding for understanding HIV transmission. Cell 131: 1044-6.
  61. Roan N.R., Starnbach M.N. 2006. Antigen-specific CD8+ T cells respond to Chlamydia trachomatis in the genital mucosa. Journal of Immunology 117:7974-79.
  62. Roan N.R., Gierahn T.M., Higgins D.E., Starnbach M.N. 2006. Monitoring the T cell response to genital tract infection. Proceedings of the National Academy of Sciences U S A 103: 12069-74.
  63. Balsara Z.R., Roan N.R., Steele L.N., Starnbach M.N. 2006. Developmental regulation of Chlamydia trachomatis Class I Accessible Protein-1, a CD8+ T cell antigen. Journal of Infectious Diseases 193:1459-63.
  64. Hess D., Liu B., Roan N.R., Sternglanz R., Winston F. 2004. Spt10-dependent transcriptional activation in Saccharomyces cerevisiae requires both the Spt10 acetyltransferase domain and Spt21. Molecular and Cellular Biology 24:135-143.
  65. D’Orazio S.E., Velasquez M., Roan N.R., Naveiras-Torres O., Starnbach M.N. 2003. The Listeria monocytogenes lemA gene product is not required for intracellular infection or to activate fMIGWII-specific T cells. Infection and Immunity 71:6721-27.