Publications

  1. Cavrois M., Hilton J.F., Roan N.R., Takeda M., Seidman D., Averbach S., Chang E., Raman N., Greenblatt R., Shacklett B.L. Smith-McCune K. Effects of the levonorgestrel-containing device, copper intrauterine device, and levonogestrel-containing oral contraceptive on susceptibility of immune cells from cervix, endometrium and blood to HIV-1 fusion measured ex vivo. 2019. PloS One, 14(8):e0221181.
  2. Skinner W.M., Mannowetz N., Lishko P.V., Roan N.R. Single-cell motility analysis of tethered human spermatozoa. 2019. Bio-protocol, 9(5): e3182.
  3. Lausten A., Bak R.O., Krapp, C., Kjaer L., Egedahl J.H., Petersen C.C., Pillai S., Tang H.Q., Uldbjerg N., Porteus M., Roan N.R., Nyegaard M., Denton P.W., Jakobsen M.R. Interferon priming is essential for human CD34+ cell-derived plasmacytoid dendritic cell maturation and function. 2018. Nature Communications, 30;9(1):3525. 
  4. Müller J.A., Harms M., Krüger F., Groß R., Joas S., Hayn M., Dietz A.N., Lippold S., von Einem J., Schubert A., Michel M., Mayer B., Cortese M., Jang K.S., Sandi-Monroy N., Deniz M., Ebner F., Vapalahti O., Otto M., Bartenschlager R., Herbeuval JP, Schmidt-Chanasit J., Roan N.R., Münch J. Semen inhibits Zika virus infection of cells and tissues from the anogenital region. 2018. Nature Communications, 9(1):2007.
  5. Röcker A., Roan N.R., Yadav J.K., Fändrich M., Münch J. 2018. Structure, function and antagonism of semen amyloids. Chemical Communications, 54(55):7557-7569.
  6. Roan N.R. Resident T cells stand up to HIV. 2018. Science Immunology Focus article. 2018. 3(24) pii: eaat6121.
  7. Martin J.W., Chen J.C., Neidleman J., Tatsumi K., Hu J., Giudice L.C., Greene W.C., Roan N.R. Potent and rapid activation of tropomyosin-receptor kinase A in endometrial stromal fibroblasts by seminal plasma. 2018. Biology of Reproduction, 99(2):336-348.
  8. Trepecar M., Khan S., Roan N.R., Chen T.H., Telwatte S., Deswal M., Pao M., Somsouk M., Deeks S.G., Hunt P.W., Yukl S., Sanjabi S. 2017. An optimized and validated method for isolation and characterization of lymphocytes from HIV+ human gut biopsies. AIDS and Human Retroviruses 33(S1):S31-S39.
  9. Cavrois M., Banerjee T., Mukherjee G., Raman N., Hussien R., Rodriguez B.A., Vasquez J., Spitzer M.H., Lazarus N.H., Jones J.J., Ochsenbauer C., McCune J.M., Butcher E.C., Arvin A.M., Sen N., Greene W.C., Roan N.R. Mass cytometric analysis of HIV entry, replication, and remodeling in tissue CD4+ T cells. 2017. Cell Reports 20(4):984-998.
  10. Roan N.R., Sandi-Monroy N., Kohgadai N., Usmani S.M., Hamil K.G., Neidleman J., Montano M., Standker L., Rocker A., Cavrois M., Rosen J., Marson K., Smith J.F., Pilcher C.D., Gagsteiger F., Sakk O., O’Rand M., Lishko P.V., Kirchhoff F., Münch J., Greene W.C. Semen amyloids participate in human spermatozoa selection and clearance. 2017. Elife 6:e24888.
  11. Neidleman J.A., Chen J.C., Kohgadai N., Müller J.A., Laustsen A., Thavachelvam K., Jang K.S., Stürzel C.M., Jones J.J., Ochsenbauer C., Chitre A., Somsouk M., Garcia M.M., Smith J.F., Greenblatt R.M., Münch J., Jakobsen M.R., Giudice L.C., Greene W.C., Roan N.R. Mucosal stromal fibroblasts markedly enhance HIV infection of CD4+ T cells. 2017. PLOS Pathogens 13(2):e1006163.
  12. LoRicco J.G., Xu C.S., Neidleman J., Bergkvist M., Greene W.C., Roan N.R.*, Makhatadze G.I.* Gallic acid is an antagonist of semen amyloid fibrils that enhance HIV-1 infection. *Co-corresponding authors. 2016. J Biol Chem, 291(27):14045.
  13. Bergman P., Roan N.R., Römling U., Bevins C.L., Münch J. Amyloid formation: functional friend or fearful foe? 2016. J Intern Med, 280(2):139.
  14. Müller J.A.*, Zirafi O.*, Roan N.R., Lee S.J., Münch J. Evaluation of EPI-X4 as a urinary peptide biomarker for diagnosis and prognosis of late acute GVHD. 2016. *Authors contributed equally to work. 2016. Bone Marrow Transplantation, 51(8):1137.
  15. Roan N.R. and Jakobsen M.R. Friend or foe: Innate sensing of HIV in the female reproductive tract. 2016. Curr HIV/AIDS Rep, 13(1):53.
  16. Camus C., Matusali G., Bourry O., Mahe D., Aubry F., Bujan L., Pasquier C., Massip P.,Ravel C., Zirafi O., Münch J, Roan N.R., Pineau C., Dejucq-Rainsford N. Comparison of the effect of semen from HIV-infected and uninfected men on CD4+ T cell infection. 2016. AIDS, 30(8):1197.
  17. Roan N.R. and Münch J. Improving preclinical models of HIV microbicide efficacy. 2015. Trends in Microbiology, 23(8):445.
  18. Chen J.C., Roan N.R. Isolation and culture of human endometrial epithelial cells and stromal fibroblasts. 2015. Bio Protocols, 5(20). Pii: 31623.
  19. Zirafi O.*, Kim K.A.*, Roan N.R.*, Kluge S.F., Müller J.A., Jiang S., Mayer B., Greene W.C., Kirchhoff F., Münch J. 2014. *Authors contributed equally to work. Semen enhances HIV infectivity and impairs the antiviral efficacy of microbicides. Science Translational Medicine, 6(262):262ra157.
  20. French K.C., Roan N.R., Makhatadze G.I. 2014. Structural characterization of semen coagulum-derived SEM1(86–107) amyloid fibrils that enhance HIV-1 infection. Biochemistry, 53(20):3267-77.
  21. Roan N.R., Liu H., Usmani S.M., Neidleman J., Müller A.J., Avila-Herrera A., Gawanbacht A., Zirafi O., Chu S., Dong M., Kumar S.T., Smith J.F., Pollard K.S., Fändrich M., Kirchhoff F., Münch J., Witkowska H.E., Greene W.C. 2014. Liquefaction of semen generates and later degrades a conserved semenogelin peptide that enhances HIV infection. Journal of Virology, 88(13):7221-7234.
  22. Roan N.R., Chu S., Liu H., Neidleman, J., Witkowska, H.E., Greene, W.C. 2014. The interaction of fibronectin with semen amyloids synergistically enhances HIV infection. Journal of Infectious Diseases, 210(7):1062-6.
  23. Chen J.C., Johnson B.A., Erikson D.W., Piltonen T.T., Barragan F., Chu S., Kohgadai N., Irwin J.C., Greene W.C., Giudice L.C., Roan N.R. 2014. Seminal plasma induced global transcriptomic changes associated with cell migration, proliferation, and viability in endometrial epithelial cells and stromal fibroblasts. Human Reproduction, 29(6):1255-70.
  24. Usmani S.M., Zirafi O.Z., Müller A.J., Sandi-Monroy N., Yadav J.K., Meier C., Weil T., Roan N.R., Greene W.C., Walther P., Nilsson K.P.R., Hammarström P., Wetzel R., Pilcher C.D., Gagsteiger F., Fändrich M., Kirchhoff F., Münch J. 2014. Direct Visualization of HIV-enhancing endogenous amyloid fibrils in human semen. Nature Communications, 5:3508.
  25. Tang Q., Roan N.R., Yamamura Y. 2013. Seminal plasma and semen amyloids enhance cytomegalovirus infection in cell culture. Journal of Virology 87(23):12583-91.
  26. Yolamanova M., Meier C., Shaytan A.K., Vas V., Bertoncini C.W., Arnold F., Zirafi O., Usmani S.M., Müller J.A., Sauter D., Goffinet C., Palesch D., Walther P., Roan N.R., Geiger H., Lunov O., Simmet T., Bohne J., Schrezenmeier H., Schwarz K., Ständker L., Forssmann W.G., Salvatella X., Khalatur P.G., Khokhlov A.R., Knowles T.P., Weil T., Kirchhoff F., Münch J. 2013. Peptide nanofibrils boost retroviral gene transfer and provide a rapid means for concentrating viruses. Nature Nanotechnology 8(2):130-6.
  27. Roan N.R., Cavrois M., Greene W.C. 2012. [Role of semen-derived amyloid fibrils as facilitators of HIV infection]. Med Sci (Paris), 28(4): 358-60.
  28. Arnold F., Schnell J., Zirafi O., Sturzel C., Meier C., Weil T., Standker L., Forssmann W.G., Roan N.R., Greene W.C., Kirchhoff F., Munch J. 2012. Naturally occurring fragments from two distinct regions of the prostatic acid phosphatase form amyloidogenic enhancers of HIV infection. Journal of Virology, 86(2)1244-9.
  29. Roan N.R., Müller A.J., Liu H., Chu S., Arnold F., Stürzel C., Walther P., Dong M., Witkowski, E., Kirchhoff, F., Münch J., Greene, W.C. 2011. Peptides released by physiological cleavage of semen coagulum proteins form amyloids that enhance HIV infection. Cell Host and Microbe 10: 541-550.
  30. Kim K.A., Yolamanova M., Zirafi O., Roan N.R., Standker L., Forssmann W.G., Burgener A., Dejucq-Rainsford N., Hahn B.H., Shaw G.M., Greene W.C., Kirchhoff F., Münch J. 2010. Semen-mediated enhancement of HIV infection is donor-dependent and correlates with the levels of SEVI. Retrovirology 7:55.
  31. Roan N.R., Sowinski, S., Münch J., Kirchhoff F., Greene, W.C. 2010. Aminoquinoline surfen inhibits the action of SEVI (semen-derived enhancer of viral infection). Journal of Biological Chemistry 285: 1861-9.
  32. Gondek D.C., Roan N.R., Starnbach M.N. 2009. T cell responses in the absence of IFN-g exacerbate uterine infection with Chlamydia trachomatis. Journal of Immunology 183: 1313-9.
  33. Roan N.R., Münch J., Arhel N., Mothes W., Neidleman J., Kobayashi A., Smith-McCune K., Kirchhoff F., Greene W.C. 2009. The cationic properties of SEVI underlie its ability to enhance HIV infection. Journal of Virology 83: 73-80 cover article.
  34. Starnbach M.N., Roan N.R. 2008. Conquering sexually transmitted diseases. Nature Reviews Immunology 8: 313-7.
  35. Grotenbreg G.M.*, Roan N.R.*, Guillen E., Meijers R., Wang, J., Bell, G.W., Starnbach, M.N., Ploegh, H.L. 2008. Discovery of CD8+ T cell epitopes in Chlamydia trachomatis infection through use of caged class I MHC tetramers. *Authors contributed equally to work. Proceedings of the National Academy of Sciences U S A. 105:3831-6.
  36. Roan N.R., Starnbach, M.N. 2008. Immune-mediated control of Chlamydia infection. Cellular Microbiology 10: 9-19.
  37. Roan N.R., Greene, W.C. 2007. A seminal finding for understanding HIV transmission. Cell 131: 1044-6.
  38. Roan N.R., Starnbach M.N. 2006. Antigen-specific CD8+ T cells respond to Chlamydia trachomatis in the genital mucosa. Journal of Immunology 117:7974-79.
  39. Roan N.R., Gierahn T.M., Higgins D.E., Starnbach M.N. 2006. Monitoring the T cell response to genital tract infection. Proceedings of the National Academy of Sciences U S A 103: 12069-74.
  40. Balsara Z.R., Roan N.R., Steele L.N., Starnbach M.N. 2006. Developmental regulation of Chlamydia trachomatis Class I Accessible Protein-1, a CD8+ T cell antigen. Journal of Infectious Diseases 193:1459-63.
  41. Hess D., Liu B., Roan N.R., Sternglanz R., Winston F. 2004. Spt10-dependent transcriptional activation in Saccharomyces cerevisiae requires both the Spt10 acetyltransferase domain and Spt21. Molecular and Cellular Biology 24:135-143.
  42. D’Orazio S.E., Velasquez M., Roan N.R., Naveiras-Torres O., Starnbach M.N. 2003. The Listeria monocytogenes lemA gene product is not required for intracellular infection or to activate fMIGWII-specific T cells. Infection and Immunity 71:6721-27.